abstract：:Halophilic and freshwater strains of cyanobacteria representing the Oscillatoriales, Nostocales, Chroococcales, and Synechococcales orders of Cyanophyta were examined to determine (i) the resistance of their cultures when suppressed by the presence of exogenous methoxylated and methylated derivatives of 2'-hydroxychal...

journal_title：Journal of biotechnology

authors： Żyszka-Haberecht B,Poliwoda A,Lipok J

更新日期：2019-03-10 00:00:00

abstract：:Approaches using metabolic engineering to produce D-1, 2, 4-butanetriol (BT) from renewable biomass in microbial systems have achieved initial success. However, due to the lack of incomplete understanding of the complex branch pathway, the efficient fermentation system for BT production was difficult to develop. Here ...

journal_title：Journal of biotechnology

authors： Gao Q,Wang X,Hu S,Xu N,Jiang M,Ma C,Yang J,Xu S,Chen K,Ouyang P

更新日期：2019-02-20 00:00:00

abstract：:Rebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymaticcatalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemicalmodification, represents a simple but effective route. Here, a class of highly stable and a...

journal_title：Journal of biotechnology

authors： Li L,Li H,Yan B,Yu S

更新日期：2019-02-10 00:00:00

abstract：:We report the design and characterization of novel fusion proteins, consisting of the F8 antibody and of murine calreticulin (Calr). The F8 antibody recognizes the alternatively-spliced ED-A domain of fibronectin, an extracellular matrix component found in most tumor types, while calreticulin has previously been descr...

journal_title：Journal of biotechnology

authors： Ziffels B,Grötsch A,Al-Bayati L,Neri D

更新日期：2019-01-20 00:00:00

abstract：:Glutathione (GSH) is an important bioactive tripeptide and widely used in food, medicine and other industries. Recently, the bifunctional glutathione synthetase, GshF, has been applied for efficient GSH production under inducible promoter. In this study, the constitutive expression of GshF from Streptococcus sanguinis...

journal_title：Journal of biotechnology

authors： Cui X,Wan J,Zhang X,Wu H,Li Z,Ye Q

更新日期：2019-01-10 00:00:00

abstract：:Formation of disulfide bonds between heavy and light chains is challenge for production of recombinant immunoglobulin G (IgG) and fragment antigen-binding (Fab). Insufficient formation of the bond influences productive yield and quality of recombinant IgG and Fab. To investigate how amino acid sequences of the first h...

journal_title：Journal of biotechnology

authors： Yusakul G,Sakamoto S,Tanaka H,Morimoto S

更新日期：2018-12-20 00:00:00

abstract：:Environmental gradient is an important common issue during scale-up process for protein production. To address the dynamic regulatory mechanism of Aspergillus niger being exposed to inhomogeneous glucose concentrations, glucose perturbation were experimented on the steady state of A. niger chemostat culture, and dynam...

journal_title：Journal of biotechnology

authors： Li C,Shu W,Wang S,Liu P,Zhuang Y,Zhang S,Xia J

更新日期：2018-12-10 00:00:00

abstract：:Vitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent ...

journal_title：Journal of biotechnology

authors： Moulas AN,Vaiou M

更新日期：2018-11-10 00:00:00

abstract：:Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...

journal_title：Journal of biotechnology

authors： Zirpel B,Kayser O,Stehle F

更新日期：2018-10-20 00:00:00

abstract：:CRISPR-Cpf1 is a type V CRISPR system that has recently been exploited for genome engineering purposes. Compared to the well-known Streptococcus pyogenes CRISPR-Cas9 system, the effector protein Cpf1 recognizes T-rich protospacer-adjacent motif (PAM) instead of G-rich PAM (used by CRISPR-Cas9), which could offer a sub...

journal_title：Journal of biotechnology

authors： Zhang J,Hong W,Zong W,Wang P,Wang Y

更新日期：2018-10-20 00:00:00

abstract：:The genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antib...

journal_title：Journal of biotechnology

authors： Serrano-Maldonado CE,Quirasco M

更新日期：2018-10-10 00:00:00

abstract：:During tumour development, macrophages are recruited to the tumour site and orientated towards an anti-inflammatory phenotype. Due to their immunosuppressive function, tumour associated macrophages (TAMs) are recognized as major components in tumour progression. Changing these macrophages to a pro-inflammatory phenoty...

journal_title：Journal of biotechnology

authors： Rodet F,Capuz A,Hara T,van Meel R,Duhamel M,Rose M,Raffo-Romero A,Fournier I,Salzet M

更新日期：2018-09-20 00:00:00

abstract：:4-vinylguaiacol, a kind of volatile phenolic compound with tobacco flavor, is widely used as a component of edible flavor and intermediate of medicine. Ferulic acid is usually used as substrate for the biosynthesis of 4-vinylguaiacol. However, the price of ferulic acid is high, leading to high production cost. In this...

journal_title：Journal of biotechnology

authors： Sun LH,Lv SW,Yu F,Li SN,He LY

更新日期：2018-09-10 00:00:00

abstract：:The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplan...

journal_title：Journal of biotechnology

authors： Chen Q,Zhang S,Xie Y

更新日期：2018-09-10 00:00:00

abstract：:For pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable ...

journal_title：Journal of biotechnology

authors： Stuible M,Burlacu A,Perret S,Brochu D,Paul-Roc B,Baardsnes J,Loignon M,Grazzini E,Durocher Y

更新日期：2018-09-10 00:00:00

abstract：:To improve the specific activity and catalytic efficiency of l-LcLDH1, an NADH-dependent allosteric l-lactate dehydrogenase from L. casei, towards phenylpyruvic acid (PPA), its directed modification was conducted based on the semi-rational design. The three variant genes, Lcldh1Q88R, Lcldh1I229A and Lcldh1T235G, were ...

journal_title：Journal of biotechnology

authors： Li JF,Li XQ,Liu Y,Yuan FJ,Zhang T,Wu MC,Zhang JR

更新日期：2018-09-10 00:00:00

abstract：:The aim of the present study was to investigate the effect of three routine drug excipients, as osmolytes, in three different concentrations, on structure, thermal stability and the activity of single-chain (sc-) tenecteplase. To see the influence of trehalose, mannitol, and sucrose on the structure, stability and func...

journal_title：Journal of biotechnology

authors： Bayat M,Gourabi H,Khammari A,Ahmad F,Saboury AA

更新日期：2018-08-20 00:00:00

abstract：:New emerging technologies delivering benefits in terms of process robustness and economy are an inevitable prerequisite for monoclonal antibody purification processes intensification. Caprylic acid was proven as an effective precipitating agent enabling efficient precipitaton of product- and process-related impurities...

journal_title：Journal of biotechnology

authors： Trapp A,Faude A,Hörold N,Schubert S,Faust S,Grob T,Schmidt S

更新日期：2018-08-10 00:00:00

abstract：:The pharmaceutical production of recombinant proteins, such as monoclonal antibodies, is rather complex and requires proper development work. Accordingly, it is essential to develop appropriate scale-down models, which can mimic the corresponding production scale. In this work, we investigated the impact of the biorea...

journal_title：Journal of biotechnology

authors： Bertrand V,Vogg S,Villiger TK,Stettler M,Broly H,Soos M,Morbidelli M

更新日期：2018-08-10 00:00:00

abstract：:Cell wall integrity signaling pathway in Saccharomyces cerevisiae is a conserved function for detecting and responding to cell stress conditions but less understood for industrial yeast. We examined gene expression dynamics for a tolerant industrial yeast strain NRRL Y-50049 in response to challenges of furfural and H...

journal_title：Journal of biotechnology

authors： Liu ZL,Wang X,Weber SA

更新日期：2018-06-20 00:00:00

abstract：:Starvation is one of the most common forms of stress experienced in the wild life. Such conditions associate the other forms of stress such as acid, heat, oxidation, and so on. Organisms acclimate to such stresses and acquire the stress tolerances, which often trade-off their growth rates. To investigate whether starv...

journal_title：Journal of biotechnology

authors： Shibata K,Obase K,Itoh K,Amemiya T

更新日期：2018-06-10 00:00:00

abstract：:Magnetic Fe3O4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were sim...

journal_title：Journal of biotechnology

authors： Su E,Meng Y,Ning C,Ma X,Deng S

更新日期：2018-04-10 00:00:00

abstract：:A broad methanotrophic community consisting of 16 different operational taxonomic units (OTUs) was detected by particulate methane monooxygenase A (pmoA) gene analyses of reactor sludge samples obtained from an industrial biogas plant. Using a cloning-sequencing approach, 75% of the OTUs were affiliated to the group o...

journal_title：Journal of biotechnology

authors： May T,Polag D,Keppler F,Greule M,Müller L,König H

更新日期：2018-03-20 00:00:00

abstract：:Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with...

journal_title：Journal of biotechnology

authors： Frohnmeyer E,Frisch F,Falke S,Betzel C,Fischer M

更新日期：2018-03-10 00:00:00

abstract：:A growing body of evidence has reinforced the central role of microbiomes in the life of sound multicellular eukaryotes, thus more properly described as true holobionts. Though soil was considered a main source of plant microbiomes, seeds have been shown to be endophytically colonized by microorganisms thus representi...

journal_title：Journal of biotechnology

authors： López JL,Alvarez F,Príncipe A,Salas ME,Lozano MJ,Draghi WO,Jofré E,Lagares A

更新日期：2018-02-10 00:00:00

abstract：:The application of biocalorimetry to fermentation processes offers advantageous insights, while being less complex compared to other, sophisticated PAT solutions. Although the general concept is established, calorimetric methods vary in detail. In this work, a special approach, called heat compensation calorimetry, wa...

journal_title：Journal of biotechnology

authors： Müller M,Meusel W,Husemann U,Greller G,Kraume M

更新日期：2018-01-20 00:00:00

abstract：:As the important chiral building block of levetiracetam, the synthesis of (S)-2-aminobutanamide has attracted a great deal of attention. The d-aminopeptidase catalyzed kinetic resolution of 2-aminobutanamide was demonstrated as an effective strategy for (S)-2-aminobutanamide production. In this study, a novel d-aminop...

journal_title：Journal of biotechnology

authors： Tang XL,Lu XF,Wu ZM,Zheng RC,Zheng YG

更新日期：2018-01-20 00:00:00

abstract：:Waste paper, a major fraction of municipal solid waste, has a potential to serve as renewable feedstock for the biorefineries of fuels, chemicals and materials due to rich in cellulose and abundant at low cost. This study evaluates the possibility of waste office paper (WOP) to serve as a potential feedstock for the b...

journal_title：Journal of biotechnology

authors： Neelamegam A,Al-Battashi H,Al-Bahry S,Nallusamy S

更新日期：2018-01-10 00:00:00

abstract：:This paper introduces a novel approach to enhance the strains' biodegradation of 4-chloronitrobenzene by utilizing the synergistic effect of the organic reductant mannitol and the substrate beef extraction. Our results demonstrate that 4-chloronitrobenzene could not be an available nitrogen source to support target st...

journal_title：Journal of biotechnology

authors： Li T,Zhang TC,He L

更新日期：2017-12-20 00:00:00

abstract：:Experiments in the life sciences often involve tools from a variety of domains such as mass spectrometry, next generation sequencing, or image processing. Passing the data between those tools often involves complex scripts for controlling data flow, data transformation, and statistical analysis. Such scripts are not o...

journal_title：Journal of biotechnology

authors： Fillbrunn A,Dietz C,Pfeuffer J,Rahn R,Landrum GA,Berthold MR

更新日期：2017-11-10 00:00:00

abstract：:The importance of RNA-based regulation is becoming more and more evident. Genome-wide sequencing efforts have shown that the majority of the DNA in eukaryotic genomes is transcribed. Advanced high-throughput techniques like CLIP for the genome-wide detection of RNA-protein interactions have shown that post-transcripti...

journal_title：Journal of biotechnology

authors： Backofen R,Engelhardt J,Erxleben A,Fallmann J,Grüning B,Ohler U,Rajewsky N,Stadler PF

更新日期：2017-11-10 00:00:00

abstract：:Collecting, curating, interlinking, and sharing high quality data are central to de.NBI-SysBio, the systems biology data management service center within the de.NBI network (German Network for Bioinformatics Infrastructure). The work of the center is guided by the FAIR principles for scientific data management and ste...

journal_title：Journal of biotechnology

authors： Wittig U,Rey M,Weidemann A,Müller W

更新日期：2017-11-10 00:00:00

abstract：BACKGROUND:In recent years, several mass spectrometry-based omics technologies emerged to investigate qualitative and quantitative changes within thousands of biologically active components such as proteins, lipids and metabolites. The research enabled through these methods potentially contributes to the diagnosis and ...

journal_title：Journal of biotechnology

authors： Pfeuffer J,Sachsenberg T,Alka O,Walzer M,Fillbrunn A,Nilse L,Schilling O,Reinert K,Kohlbacher O

更新日期：2017-11-10 00:00:00

abstract：:Enzyme prodrug therapies hold potential as a targeted treatment option for cancer patients. However, off-target effects can be detrimental to patient health and represent a safety concern. This concern can be alleviated by including a failsafe mechanism that can abort the therapy in healthy cells. This feature can be ...

journal_title：Journal of biotechnology

authors： Gaynor AS,Chen W

更新日期：2017-10-20 00:00:00

abstract：:Bacillus flexus KLBMP 4941 is a halotolerant endophyte isolated from the halophyte Limonium sinense. This strain can improve host seedling growth under salt stress conditions. We here report the complete genome information of endophyte KLBMP 4941. It has a circular chromosome and two plasmids for a total genome 4,104,...

journal_title：Journal of biotechnology

authors： Wang TT,Ding P,Chen P,Xing K,Bai JL,Wan W,Jiang JH,Qin S

更新日期：2017-10-20 00:00:00

abstract：:Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical syn...

journal_title：Journal of biotechnology

authors： Kim JW,Yoon HC,Kwon SJ,Lee BY,Lee PC

更新日期：2017-10-10 00:00:00

abstract：:The global occurrence of prostate cancer with a range of patient outcome has prompted various investigators to explore novel molecular biomarkers that can precisely detect and track this type of cancer severity. Several studies suggest that micro-RNAs have emerged to act as a new largely unexplored class of biomarkers...

journal_title：Journal of biotechnology

authors： Wani S,Kaul D,Mavuduru RS,Kakkar N,Bhatia A

更新日期：2017-10-10 00:00:00

abstract：:Sugarcane bagasse (SCB) is an abundant, renewable and inexpensive agricultural byproduct for the production of biofuel and other biobased products. To effectively saccharify SCB with cellulases, combination with dilute alkali salts Na2SO3/Na3PO4 (0.4% Na3PO4, 0.03% Na2SO3) at 7.5% sulfidity and hot water (DASHW) in "o...

journal_title：Journal of biotechnology

authors： Jiang CX,He YC,Chong GG,Di JH,Tang YJ,Ma CL

更新日期：2017-10-10 00:00:00

abstract：:The cyclic amino acid ectoine is a compatible solute serving as a protective substance against osmotic stress. Ectoine finds various applications due to its moisturizing effect. To avoid the disadvantages of the prevailing so-called "bacterial milking ectoine production process" caused by the high salt concentration, ...

journal_title：Journal of biotechnology

authors： Pérez-García F,Ziert C,Risse JM,Wendisch VF

更新日期：2017-09-20 00:00:00

abstract：:Steady state studies in a chemostat enable the control of microbial growth rate at defined reaction conditions. The effects of bacteriophage M13 infection on maximum growth rate of Escherichia coli JM109 were studied in parallel operated chemostats on a milliliter-scale to analyze the steady state kinetics of phage pr...

journal_title：Journal of biotechnology

authors： Kick B,Behler KL,Severin TS,Weuster-Botz D

更新日期：2017-09-20 00:00:00